Phase I trial of chimeric anti-GPC3 scFv-CD3ε engineered T cells (CT0180) in patients with advanced hepatocellular carcinoma.

person Yi Zheng Department of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China info_outline Yi Zheng, Qihan Fu, Qingwei Zhao, Lulu Liu, Zhou Tong, Hangyu Zhang, Peng Zhao, Weijia Fang, Xudong Zhu, Wanwan Gao, Miya Wang, Daijing Yuan, Huamao Wang, Zonghai Li, Tingbo Liang

Authors person Yi Zheng Department of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China info_outline Yi Zheng, Qihan Fu, Qingwei Zhao, Lulu Liu, Zhou Tong, Hangyu Zhang, Peng Zhao, Weijia Fang, Xudong Zhu, Wanwan Gao, Miya Wang, Daijing Yuan, Huamao Wang, Zonghai Li, Tingbo Liang Organizations Department of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China, Department of Clinical Pharmacy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China, Department of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China, CARsgen Therapeutics Ltd., Shanghai, China, CARsgen Therapeutics Ltd., Co., Shanghai, China, CARsgen Therapeutics Co., Ltd, Shanghai, China, CARsgen Therapeutics, Inc, Houston, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China Abstract Disclosures Research Funding Pharmaceutical/Biotech Company CARsgen Therapeutics Ltd Background: Hepatocellular carcinoma (HCC) is associated with poor prognosis and high mortality. Glypican-3 (GPC3) is a heparan sulfate proteoglycan overexpressed in 70-80% of HCC and is considered a potential therapeutic target for HCC. We modified T cells with a fusion protein of anti-GPC3 single-chain fragment variable (scFv) linked to CD3ε, which incorporates into the native T cell receptor/CD3 complex forming chimeric anti-GPC3 scFv-CD3ε engineered T cells (CT0180). Preclinically, CT0180 showed competitive antitumor activity but lower cytokine release compared to 28ζ or BBζ chimeric antigen receptor T cells. Methods: This is an open-label, dose-escalation/exploration phase I study to investigate CT0180 in patients with GPC3-positive advanced HCC (NCT04756648). The study objectives are to evaluate the safety, preliminary efficacy, and cellular pharmacokinetics of CT0180. Eligible patients underwent sequential apheresis, lymphodepletion, and cell infusion. Antiangiogenic drug monotherapy was allowed after apheresis as bridging therapy. Lymphodepletion regimen comprised fludarabine 25 mg/m 2 and cyclophosphamide 300 mg/m 2 daily for 3 days. Five dose levels (DLs, range 10×10 6 -600×10 6 cells) with up to 3 cycles were explored using i3 + 3 design, and intra-patient dose-escalation was allowed. Data are reported as of 01-Feb-2023. Results: From Feb-2021 to Jul-2022, 7 patients with hepatitis B virus-related HCC were treated with CT0180 (one patient each at 10×10 6 and 30×10 6 DLs, 3 at 100×10 6 DL, and 2 at 300×10 6 DL). The median age was 46 (28–74) years. Patients had prior 2 lines or more systemic therapy, with at least one antiangiogenic tyrosine kinase inhibitor and/or anti-PD-1/PD-L1 immunotherapy. Most common grade 3-4 adverse events were hematologic toxicities, ie lymphopenia and neutropenia, which were considered to be related to lymphodepletion. No dose-limiting toxicities, immune effector cell-associated neurotoxicity syndrome, or treatment-related deaths occurred. Grade 1 cytokine release syndrome was observed in 6 patients; tocilizumab was given in one patient and no glucocorticoids were used. All 7 patients were evaluable for efficacy, in which 2 achieved partial response (PR) (30×10 6 and 300×10 6 DL) and 3 achieved stable disease (SD) (10×10 6 , 100×10 6 and 300×10 6 DL). One patient sustained PR for 6.7 months, and one patient sustained SD for 6.1 months with follow-up ongoing. Median follow-up time was 15.9 months, and median overall survival was 11.6 months with 3 patients alive at last follow-up. CT0180 transgene copy number ranged 47–4487 copies/μg genomic DNA and peaked on either D3 or D7. Interleukin-6 increased and peaked on D1 after CT0180 infusion in most patients. Conclusions: CT0180 demonstrated manageable safety profile and promising antitumor potential. Further exploration of CT0180 in HCC is needed. Clinical trial information: NCT04756648.

Clinical