Abstract
Association between cervical immune markers and cervical high-risk HPV infections.
Author
Sally Nneoma Adebamowo
Department of Epidemiology and Public Health and Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD
info_outline
Sally Nneoma Adebamowo, Oluranti Ayotunde Famooto, Clement Adebayo Adebamowo
Full text
Authors
Sally Nneoma Adebamowo
Department of Epidemiology and Public Health and Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD
info_outline
Sally Nneoma Adebamowo, Oluranti Ayotunde Famooto, Clement Adebayo Adebamowo
Organizations
Department of Epidemiology and Public Health and Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD, Institute of Human Virology Nigeria, Abuja Municipal, Nigeria
Abstract Disclosures
Research Funding
U.S. National Institutes of Health
U.S. National Institutes of Health, American Cancer Society Research Scholar Grant RSG-22-079-01-CSCT
Background:
Evidence suggests that human papillomavirus (HPV) infection of the cervix, alters local immune markers. The aim of this study was to comprehensively evaluate the types and concentration of cervical cytokines and chemokines in HPV-negative compared to HPV-positive women in sub-Saharan African.
Methods:
The study population was 275 women enrolled in the African Collaborative Center for Microbiome and Genomics (ACCME) HPV cohort study. The concentration of 27 chemokines, cytokines and growth factors was quantified from cervical samples, using multiplexed bead-based immunoassays. HPV types were characterized using SPF
25
/LiPA
10
. Regression models were used to estimate the association between each immune marker and HPV infection. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated for fold-change in immune marker levels divided into quartiles, using the lowest quantile as a reference category.
Results:
The mean (±SD) age of the women was 41 (±8) years. Some, 49% (134/275) were HPV negative and 51% (141/275) were HPV positive. Of the HPV positive women, 39% (55/141) had low-risk HPV (lrHPV) types and 93% (131/141) had high-risk HPV (hrHPV) types. Compared to HPV negative women, lrHPV-positive women had significantly higher median concentration of IFN-g, IL-2, IL-4, IL-7, IL-10 and IL-13. While hrHPV-positive women had significantly lower median concentration of GMCSF, interleukin (IL)-1RA and MCP-1; and higher median concentration of interferon-g (IFN-g), IL-b, IL-6, IL-7, IL-8, IL-9, IL-10, IL-13, IL-15 and MCP-1b, compared to HPV negative women. Women had significantly higher odds of lrHPV infection if they were in the highest quartile for IL-2, IL-4, IL-5, IL-7, IL-10, IL-12p70 and IL-13. While women had significantly lower odds of hrHPV infection if they were in the highest quartile for IL-IRA (OR 0.16, 95% CI: 0.07, 0.34) or MCP-1 (OR 0.39, 95% CI: 0.18, 0.83), and significantly higher odds of hrHPV infections if they were in the highest quartile for eotaxin, IFN-g, IL-1b, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-13, IL-15, MIP-1a, MIP-1b or TNFa.
Conclusions:
Consistent with findings from other populations, higher concentration of pro-inflammatory immune markers in the cervix were associated with higher odds of high-risk HPV infections among African women.
2 organizations
20 drugs
20 targets
Organization
University of Maryland School of MedicineOrganization
Institute of Human Virology NigeriaDrug
IFN-gDrug
IL-2Drug
IL-4Drug
IL-7Drug
IL-10Drug
IL-13Drug
GMCSFDrug
IL-1RADrug
MCP-1Drug
IL-bDrug
TocilizumabDrug
IL-8Drug
IL-9Drug
MCP-1bDrug
IL-12p70Drug
IL-1bDrug
MIP-1aDrug
MIP-1bDrug
TNFAIP6Target
MCP-1bTarget
IL-6Target
IL-4 receptor (IL-4R)Target
IL-2Target
IL-9Target
IL-7Target
GMCSFTarget
IL-13Target
MCP-1Target
IL-8Target
IL-1RATarget
IL-bTarget
IL-1bTarget
MIP-1bTarget
IL-10Target
IFN-gammaTarget
TNFaTarget
MIP-1aTarget
IL-12p70Target
IL-15