12/15-LIPOXYGENASE ORCHESTRATES THE CLEARANCE OF APOPTOTIC CELLS AND MAINTAINS IMMUNOLOGICAL TOLERANCE

Background: The coordinated and non-inflammatory phagocytosis of apoptotic cells is crucial to maintain immunological tolerance. During inflammation, however, ingestion of apoptotic material by inflammatory phagocytes can provoke a break in self-tolerance. Hence, and though poorly understood, mechanisms governing the sorting of apoptotic cells into distinct and differentially-activated subsets of phagocytes are essentialto prevent autoimmunity. Objectives: To understand mechanisms, which govern the sorting of apoptotic cells into differentially activated subsets of macrophages and thereby contribute to the maintenance of tolerance. Methods: We determined the role of the enzyme 12/15-lipoxygenase (12/15-LO) during the clearance of apoptotic cells by analyzing the uptake of apoptotic cells by distinct macrophage subpopulations in wild-type and 12/15-LO-deficient mice. Moreover, we performed the pristane-model of murine lupus erythematosus to determine the role of this enzyme during the maintenace of immunological tolerance in vivo. Results: Here we identify the enzyme 12/15-lipoxygenase (12/15-LO) as a crucial factor orchestrating the clearance of apoptotic cells under inflammatory conditions. During peritonitis, the ingestion of apoptotic cells is confined to a distinct population of alternatively-activated, 12/15-LO-expressing resident macrophages (resMΦ). Deletion of 12/15-LO changed this pattern and neighbouring inflammatory macrophages (infMΦ) start to engulf apoptotic cells. We hypothesized that resMΦ exert a paracrine and 12/15-LO-mediated inhibitory activity on the phagocytic capacity of infMΦ and identified oxidation products of phosphatidylethanolamine (oxPE) as the corresponding mediators. oxPE is generated by the action of 12/15-LO in resMΦ and consequently exposed on the macrophages' surfaces. Here, oxPE binds, blocks and scavenges soluble MFG-E8 protein and thereby selectively blocks the major pathway involved in the uptake of apoptotic cells into infMΦ. In turn, we observed a break in self-tolerance and lupus-like autoimmune disease in aged 12/15-LO-deficient mice. These mice displayed spontaneous production of autoantibodies and glomerulonephritis, which both exacerbated after apoptotic challenge in the pristane-induced model of experimental murine lupus. Conclusions: Our data point towards a so far unrecognized role for enzymatic lipid oxidation during the maintenance of self-tolerance and identify a mechanism, which orchestrates the cell- and context-specific uptake of antigens by different subsets of phagocytes, imposing a new paradigm in our understanding of the clearance of apoptotic cells. Disclosure of Interest: None DeclaredCitation: Annals of the Rheumatic Diseases, volume 70, supplement 3, year 2011, page 673Session: SLE, Sjögren's and APS – etiology, pathogenesis and animal models (Poster Presentations )