ANTIOXIDANT STATUS AND OXIDATIVE STRESS IN PATIENTS WITH BEHÇET'S DISEASE

Background: It was shown that many antioxidants decrease in Behçet's disease (BD). Total antioxidant response (TAR), whose measurement method has been recently specified and developed, represents the total antioxidant properties of all antioxidants. Paraoxonase (PON), arylesterase (ARE), free sulphydryl (SH) groups and ceruloplasmin (CP) are enzymes or proteins with antioxidant characteristics. Lipid hydroperoxides (LOOHs) are byproduct of lipid peroxidation.Objectives: To determine the levels of LOOHs, total peroxide (TP) and SH, the activities of PON1, ARE and CP and the antioxidant capacity with TAR in active and inactive BD patients.Methods: This study included 21 BD cases (10 active and 11 inactive) and 23 healthy volunteers. The LOOHs and SH levels and PON1, ARE and CP activities were determined. TAR and TP were studied using appropriate methods.Results: LOOHs and TP levels were found to be significantly high, while TAR and SH levels and PON1, ARE and CP activities were observed as significantly low in the BD, when compared with the control. There was a significant negative correlation between the level of LOOHs and the activity of ARE in the BD (r=-0.46, p<0.05). TAR level was negatively correlated with TP level both in the BD (r=-0.578, p<0.01) and the control (r=-0.469, p<0.05) groups. There was not any significant difference between active and inactive BD groups in terms of TAR and TP. An increase in the level of LOOHs and a decrease in the activities of PON1, ARE and CP were observed in the active BD group when compared with the inactive BD group, but differences were not statistical significant. Laboratory findings of the patient and the control groups BD (n=21) Controls (n=23) PON1 (U/L) 91.8±20.1† 149.1±21.32 ARE (U/L) 337.8±48.8† 393.5±42.2 SH (mmol/L) 0.33±0.017† 0.43±0.012 CP (U/L) 172±27.2† 211±29.7 TAR (mmol Trolox eq./L) 1.04±0.07† 1.16±0.08 LOOHs (μmol H2O2 Eq/L) 9.9±3.2† 5.99±1.01 TP (μmol H2O2/L) 13.4±4.5† 7.72±1.86 †p<0.001. Conclusion: Results of the study indicate that the oxidant/antioxidant balance is impaired in BD. PON1 and ARE possess anti-atherosclerotic characteristics in addition to antioxidant ones. Accordingly, development of atherosclerosis in addition to tissue injury in BD seems to be inevitable due to the decrease in antioxidant levels resulting from increased oxidative stress. TAR is an appropriate method that can be used to determine the antioxidant capacity in BD.References: 1. Orem A, et al. J Dermatol Sci. 1997; 16(1): 11-6.2. Kokcam I, et al. Clin Biochem. 2002; 35(8): 633-9.3. Erel O. Clin Biochem 2004; 37(2): 112-9.4. Harma M, et al. Swiss Med Wkly 2003; 133: 563-6.Citation: Ann Rheum Dis, volume 66, supplement II, year 2007, page 76Session: Abstract Session: Vasculitis – the spectrum of vasculitides