Genomic evolution of patients with myeloid neoplasms and known antecedent clonal hematopoiesis.

person Julie Braish The University of Texas MD Anderson Cancer Center, Houston, TX info_outline Julie Braish, Ziyi Li, Sanam Loghavi, Guillermo Montalban-Bravo, Koji Sasaki, Koichi Takahashi, Courtney Denton Dinardo, Danielle Hammond, Mahesh Swaminathan, Alexandre Bazinet, Virginia Olivia Volpe, Lachelle Dawn Weeks, Hagop M. Kantarjian, Guillermo Garcia-Manero, Kelly Sharon Chien

Authors person Julie Braish The University of Texas MD Anderson Cancer Center, Houston, TX info_outline Julie Braish, Ziyi Li, Sanam Loghavi, Guillermo Montalban-Bravo, Koji Sasaki, Koichi Takahashi, Courtney Denton Dinardo, Danielle Hammond, Mahesh Swaminathan, Alexandre Bazinet, Virginia Olivia Volpe, Lachelle Dawn Weeks, Hagop M. Kantarjian, Guillermo Garcia-Manero, Kelly Sharon Chien Organizations The University of Texas MD Anderson Cancer Center, Houston, TX, Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, TX, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA Abstract Disclosures Research Funding No funding sources reported Background: Clonal hematopoiesis (CH) refers to the clonal expansion of hematopoietic stem cells (HSCs) with one or more somatic mutations in the absence of a hematologic malignancy. Although it is associated with normal aging, CH confers an increased risk of several comorbidities and hematologic malignancies, including myeloid neoplasms (MNs). However, the outcomes of patients with CH that transformed to MN are unknown. Here, we aim to evaluate the clinicopathologic characteristics and survival outcomes of patients with MN with known antecedent CH. Methods: We retrospectively evaluated patients seen at a tertiary cancer center from May 2016 to July 2023 with myelodysplastic syndromes (MDS), chronic myelomonocytic leukemia (CMML), or acute myeloid leukemia (AML) and identified those who had CH prior to MN diagnosis. At the time of MN transformation, genomic data was extracted from whole bone marrow aspirate samples and subject to 81-gene target PCR-based sequencing using a next-generation sequencing platform. Results: A total of 36 patients were identified; at the time of CH diagnosis, 79% had known cardiovascular comorbidities and 81% had a prior diagnosis of non-myeloid malignancies for which 72% received prior chemotherapy and 25% radiation. According to the Clonal Hematopoiesis Risk Score (CHRS), 22% were low, 56% deemed intermediate, and 22% at high risk of transformation to MNs. The most frequently observed CH-associated mutations were TET2 (41%), TP53 (38%), IDH2 (16%), EZH2 (16%), ASXL1 (13%), and DNMT3A (13%); 69% had more than one somatic mutation, and 61% had normal cytogenetics. The median time to transformation to MN was 15 months, 26 months, and 24 months for MDS (n=28), CMML (n=2), and AML (n=6) patients, respectively (p=0.72). The median overall survival from MN diagnosis was 54 months, not reached, and 2 months for MDS, CMML, and AML, respectively (p=0.0015). We further delved into the genomic evolution from CH to MN. In the 6 pts who transformed to AML, 4 had a TP53 mutation at CH with expansion of the clone at MN transformation, 2 acquired NPM1 mutations and 1 acquired FLT3 mutations. Both pts who progressed to CMML had expansion of their underlying CH clones (KRAS and ZRSR2). In those who developed MDS, 22 pts had expansion of their CH-mutated clone at the time of MN, most frequently in TET2 (36%), IDH2 (18%), and TP53 (14%); 5 pts acquired new driver mutations outside of their CH clone; and 1 pt lost their CH clone with no new somatic mutations detected. Conclusions: Individuals with CH are at risk of progressing to MN, especially with external selective pressures like antineoplastic therapy that are not accounted for in prognostic systems like CHRS. Patients often have underlying high-risk clones at the time of CH diagnosis that expand at the time of MN, though some patients acquired new driver mutations.