Comparison of SARS-CoV-2, AdenoVIRus and Rhinovirus Infection of the Respiratory Epithelium in CHILdren vs. the eLDerly

CHUBX 2021/43

The objective of the VIRCHILLD project is to identify age-related modifications of the bronchial epithelium physiology that account for differences in the response and susceptibility to respiratory viruses. Epidemiology and cell-based data show that respiratory virus infections differentially affect children, adults or the elderly populations. The current worldwide pandemic of SARS-CoV-2 clearly highlighted this notion with a large part of the deaths occurring in the elderly population and very few deaths amongst children. This may be linked to a decreased transmission and/or viral load with SARS-CoV-2 in children compared to adults and elderly. Less in the public eye is the observation that other major respiratory virus targeting the bronchial epithelium (BE) such as rhinovirus (RV) and adenovirus (AdV) cause important clinical feature in children and have a much lower incidence in adults and the elderly populations, which is the opposite to the situation with SARS-CoV-2. Based on this remarkable discrepancy between respiratory viruses the investigators hypothesize that intrinsic age-controlled properties of the respiratory epithelium under resting physiological conditions determine virus susceptibility and virus propagation.

2022-10-28

2025-04-28

2025-04-28

Recruiting

200

Inclusion Criteria: For pediatric patients requiring research-specific bronchial brushing: * Child under 18 years old, * Requiring bronchoscopy as part of routine care * Having informed and signed consent from the holders of parental authority For pediatric patients whose bronchial brushing will be carried out as part of their care and for those whose surgical waste will be collected for research: * Child under 18 years old, * Requiring as part of routine care bronchoscopy and brushing or bronchial fibroscopy under general anesthesia or surgical resection/lobectomy * Having expressed their non-opposition to their child's participation in research For adult patients: * Man or woman * Aged 18 or over * Requiring as part of the care, thoracic surgery such as lobectomy, pneumonectomy or lung transplantation in the Thoracic Surgery Department of Haut Lévêque du Chu Hospital in Bordeaux or inclusion in the TUBE study, described below. * having received an information note and not having objected to their participation in the research Exclusion Criteria: * No affiliation or non-beneficiary of a social security system * During a period of relative exclusion compared to another protocol * Patient born before 36 weeks of amenorrhea * Patient with a documented history of pulmonary fibrosis, primary pulmonary hypertension, cystic fibrosis, pulmonary malformation or chronic viral infections (hepatitis, HIV). * Patient with any dental, nasopharyngeal or bronchial infection with fever (\> 38°C) requiring systemic antibiotics in the previous 4 weeks

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2024-01-25