Clinical trial
Allogeneic Hematopoietic Cell Transplantation Using α/β+ T-lymphocyte Depleted Grafts From HLA Mismatched Donors
Name
18-224
Description
This study is being done to learn whether a new method to prevent rejection between the donor immune system and the patient's body is effective.
Trial arms
Trial start
2018-07-25
Estimated PCD
2024-03-06
Trial end
2024-03-06
Status
Terminated
Phase
Early phase I
Treatment
Hyperfractionated total body irradiation
Hyperfractionated TBI is administered by a linear accelerator at a dose rate of \<20 cGy/minute. Doses of 125 cGy/fraction are administered at a minimum interval of 4 hours between fractions, three times/day for a total of 11 or 12 doses (1,375 or 1,500 cGy) over 4 days (days -9 through -6).
Arms:
Radiation, Thiotepa & Cyclophosphamide
Thiotepa
Thiotepa 5 mg/kg IV
Arms:
Clofarabine, Thiotepa & Melphalan, Radiation, Thiotepa & Cyclophosphamide
Cyclophosphamide
Cyclophosphamide 60 mg/kg IV
Arms:
Radiation, Thiotepa & Cyclophosphamide
Busulfan
Busulfan (adult/ped dose)
Arms:
Busulfan, Fludarabine & Melphalan
Fludarabine
Fludarabine 25 mg/m2 IV
Arms:
Busulfan, Fludarabine & Melphalan
Melphalan
Melphalan 70 mg/m2 IV
Arms:
Busulfan, Fludarabine & Melphalan, Clofarabine, Thiotepa & Melphalan
Clofarabine
Clofarabine 20-30 mg/m2 IV
Arms:
Clofarabine, Thiotepa & Melphalan
HPC(A) stem cell allograft
All patients will receive anti-thymocyte globulin based conditioning followed by a G-CSF mobilized, peripheral blood hematopoietic progenitor cell HPC(A) product depleted of TCR-α/β+ Tlymphocytes using the CliniMACS system.
Arms:
Busulfan, Fludarabine & Melphalan, Clofarabine, Thiotepa & Melphalan, Radiation, Thiotepa & Cyclophosphamide
Rituximab
Rituximab 200 mg IV flat dose
Arms:
Busulfan, Fludarabine & Melphalan, Clofarabine, Thiotepa & Melphalan, Radiation, Thiotepa & Cyclophosphamide
Rabbit antithymocyte globulin
Rabbit antithymocyte globulin dosing per nomogram. This dynamic nomogram is based on absolute lymphocyte count at the start of conditioning and can result in either 2 or 3 day ATG administration. If a patient requires 2 day administration the subjequent chemotherapies may be moved forward by one day at the treating physician's discretion.
Arms:
Busulfan, Fludarabine & Melphalan, Clofarabine, Thiotepa & Melphalan, Radiation, Thiotepa & Cyclophosphamide
Size
9
Primary endpoint
the number of incidences of grade 3-4 acute GVHD
2 years
Eligibility criteria
Subject Inclusion Criteria:
* Patients with any of the following hematologic malignancies who are considered to be eligible for allogeneic transplantation:
* Acute lymphoid leukemia (ALL) in first complete remission (CR1) with high risk for relapse including:
* Detectable minimal residual disease by either multicolor flow cytometry or by genomic assay after initial induction therapy
* t(9;22) or detected BCR-ABL1 translocation by genomic methodologies
* BCR-ABL1-Like B-ALL \[23\] including mutations of IKZF1 or CRLF2
* Translocations or mutations involving 11q23 (MLL) gene.
* Hypodiploid karyotype
* Deletion of 9p
* Loss of 17p or TP53 mutation
* T-lymphocyte lineage antigen expression (T-ALL)
* Prior CNS or other extramedullary involvement
* WBC count ≥ 100,000 cells/μL at diagnosis
* Acute biphenotypic or bilineal leukemia in CR1
* Acute myeloid leukemia (AML) in CR1 with
* Detectable minimal residual disease (MRD) by either multicolor flow cytometry or by genomic assay after initial induction therapy
* In the absence of MRD any intermediate or high risk features according to the European LeukemiaNet 2017 guidelines indlucing:
* Mutated FL T3-ITD or FL T3-TKD
* Cytogenetic abnormalities not classified as favorable
* Cytogenetic abnormalities associated with myelodysplastic syndrome including abnormalities of chromosome 5, 7, or 17p
* Complex karyotype or monosomal karyotype
* t(9;11)(p21.1;q23.3); MLL-KMT2A or other rearrangements of KMT2A
* t(9;11); BCR-ABL1
* Inversions or translocations of chromosome 3
* T(6;9)(p23;q34.1); DEK-NUP214
* Somatic mutation of RUNX1, ASX1 or TP53
* Extramedullary involvement
* WBC count ≥100,000 cells/μL at diagnosis
* Relapsed acute leukemia with ≤ 5% blasts in the bone marrow prior to transplantation (i.e. CR2 or greater).
* Myelodysplastic syndrome, myeloproliferative neoplasms, or MDS/MPN overlap syndrome with ≤ 10% blasts and at least one of the following:
* Revised International Prognostic Scoring System risk score of INT, HIGH, or VERY HIGH at the time of transplant evaluation.
* Life-threatening cytopenias
* Karyotype or genomic changes that indicate high risk for progression to acute myelogenous leukemia, including abnormalities of chromosome 7 or 3, mutations of TP53, or complex or monosomal karyotype.
* Therapy related disease or disease evolving from other malignant processes.
* Chronic myelomonocytic leukemia (CMML) with ≤ 10% blasts prior to transplantation.
* Chronic myeloid leukemia (CML) meeting one of the following criteria:
* Failed or are intolerant to BCR-ABL tyrosine kinase inhibitors.
* CML with BCR-ABL mutation consistent with poor response to tyrosine kinase inhibition (e.g. T351I mutation).
* CML with accelerated or blast phase with \<10% blasts after therapy.
* Chronic lymphocytic leukemia (CLL) with high risk disease as defined by the EBMT consensus criteria
* Hodgkin lymphoma meeting both of the following criteria:
* Responding to therapy prior to enrollment
* Relapse after autologous bone marrow transplant or are ineligible for autologous bone marrow transplant.
°Non-Hodgkin lymphoma meeting both of the following criteria:
* Responding to therapy prior to enrollment.
* Relapse after prior autologous bone marrow transplant or are ineligible for autologous bone marrow transplant.
* Patients aged from birth through 65 years old are eligible.
* Patients must have Karnofsky/Lanksy performance status ≥70%.
* Cardiac left ventricular ejection fraction ≥50% at rest.
* Serum bilirubin ≤ 2 mg/dL. Patients with Gilbert's disease or ongoing hemolytic anemia are acceptable if the direct bilirubin is ≤ 2 mg/dL.
* AST and ALT ≤ 2.5 x ULN unless thought to be disease related
* Estimated or measured creatinine clearance \> 50 mL/min/1.73 m\^2 body surface area.
* Adult patients and pediatric patients capable of performing pulmonary function studies must have hemoglobin adjusted pulmonary DLCO ≥50% of predicted.
Subject Exclusion Criteria:
* Persons with a HLA matched sibling donor or a 8/8 allele level HLA-matched unrelated donor.
* Female patients who are pregnant or breast-feeding.
* Persons with an infection that is not responding to antimicrobial therapy.
* Persons who are seropositive for HIV.
* Persons with active/detectable central nervous system malignancy.
* Persons who do not meet the age and organ function criteria specified above.
* Presence of psychiatric or neurologic disease, or lack of social support that limits the patient's ability to comply with the treatment protocol including supportive care, followup, and research tests.
* Prior allogeneic hematopoietic cell transplantation are ineligible.
* Patients with history of other malignancy within 5 years of study therapy are ineligible with the following exceptions: Low grade prostate cancer (Gleason's ≤6) treated with curative intent, breast ductal carcinoma in situ treated with curative intent, or nonmelanomatous skin carcinomas.
Donor Inclusion and Exclusion Criteria:
* Partially HLA-matched unrelated volunteers (allele level matched at 6-7 of 8 HLA loci: -A, -B, -C, and -DRB1) are eligible.
* Related, haploidentical donors are eligible.
* Able to provide informed consent to the donation process
* Meet standard criteria for donor collection as defined by the National Marrow Donor Program Guidelines.
Protocol
{'studyType': 'INTERVENTIONAL', 'phases': ['PHASE2'], 'designInfo': {'allocation': 'NON_RANDOMIZED', 'interventionModel': 'PARALLEL', 'interventionModelDescription': 'Participants will receive one of three myeloablative conditioning regimens followed by a Alpha/beta+ T-cell depleted peripheral blood stem cell product and short course tacrolimus. Donors are HLA mismatched unrelated adults or haploidentical family members.', 'primaryPurpose': 'TREATMENT', 'maskingInfo': {'masking': 'NONE'}}, 'enrollmentInfo': {'count': 9, 'type': 'ACTUAL'}}
Updated at
2024-03-12
1 organization
Organization
Memorial Sloan Kettering Cancer Center